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Purpose(Empty Backbone) expresses sgRNA under U6d promoter
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64249 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLR-NN
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Backbone manufacturerAdam Bogdanove, Daniel Voytas lab
- Backbone size (bp) 2568
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Modifications to backbonezebrafish U6 promoter (U6d) directs a sgRNA expression. BsaI sites were added on both ends of U6d:sgRNA cassettes by PCR and PCR products were inserted into the ApaI/XbaI sites of pLR-NN from the Golden Gate TALEN kit.
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pU6d:sgRNA#5 was a gift from Wenbiao Chen (Addgene plasmid # 64249 ; http://n2t.net/addgene:64249 ; RRID:Addgene_64249) -
For your References section:
Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs. Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess SM, Patton JG, Chen W. Genetics. 2015 Apr 8. pii: genetics.115.176917. 10.1534/genetics.115.176917 PubMed 25855067
Map uploaded by the depositor.
