Purpose(Empty Backbone) destination vector for five U6x:sgRNA cassettes
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64243||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 7083
Modifications to backboneOne BsaI site in ampicillin sequence is mutated. Two BsaI sites containing adaptors with appropriate overhang (CAGT and GAAC) were sequentially inserted into the NsiI/XhoI and KpnI sites of pDestTol2LC.
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Copy numberHigh Copy
- Cloning method Restriction Enzyme
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGGDestTol2LC-5sgRNA was a gift from Wenbiao Chen (Addgene plasmid # 64243 ; http://n2t.net/addgene:64243 ; RRID:Addgene_64243)
For your References section:Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs. Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess SM, Patton JG, Chen W. Genetics. 2015 Apr 8. pii: genetics.115.176917. 10.1534/genetics.115.176917 PubMed 25855067