Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #132431)


Item Catalog # Description Quantity Price (USD)
Plasmid 132431 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5230
  • Total vector size (bp) 5981
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    Serratia marcescens nuclease (codon optimized)
  • Alt name
    NucA (Uniprot: P13717)
  • Species
  • Insert Size (bp)
  • Promoter T7
  • Tag / Fusion Protein
    • None

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Target gene is expressed from a T7 promoter so DE3 strain must be used for protein expression. The target is insoluble and can be refolded using the protocol from Friedhoff et al. A procedure for renaturation and purification of the extracellular Serratia marcescens nuclease from genetically engineered Escherichia coli. Protein Expr Purif. 1994 Feb;5(1):37-43.

The nuclease is similar to Benzonase in that it is a potent endonuclease capable of degrading single and double stranded RNA and DNA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSm-Nuclease was a gift from Mark Arbing (Addgene plasmid # 132431 ; ; RRID:Addgene_132431)