177,148 results
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Plasmid#170451PurposeExpress SARS-CoV-2 B.1.1.7 (UK strain / alpha strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 (UK strain) pseudotyped virus production.DepositorInsertSARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, UK strain (B.1.1.7))
TagsNAExpressionMammalianMutationHY69-70del, Y144del, N501Y, A570D, D614G, P681H, …PromoterCMVAvailable SinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2
Plasmid#158653Purposemaking transgenics expressing a UAS driven mCherry and NTR 2.0DepositorInsert5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
UseTol2-based transgenic expression in zebrafish/oth…MutationF70A/F108YAvailable SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hChR2(H134R)-EYFP (AAV PHP.eB)
Viral Prep#26973-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-hSyn-hChR2(H134R)-EYFP (#26973). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-EYFP plasmid DNA. hSyn-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEYFPAvailable SinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-Bst LF-6xHis
Plasmid#159148Purposeexpresses His-tagged large fragment of Bst polymeraseDepositorInsertlarge fragment of Geobacillus stearothermophilus DNA polymerase I
TagsHisExpressionBacterialAvailable SinceSept. 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV-2 NSP13
Plasmid#141266PurposeGateway-compatible Entry vector, with insert of NSP13 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertNSP13 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable SinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin
Plasmid#134868PurposeExpresses EGFP-tagged lysosomal markerDepositorInsertLysosomal-associated membrane protein 1 (LAMP1 Human)
UseLentiviralTagsGFPExpressionMammalianPromoterEF1aAvailable SinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro
Plasmid#138152PurposeExpresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleusDepositorInserteGFP-MCS
UseLentiviralTags2PEST AND NLSAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-cGAS-HA
Plasmid#130910PurposeExpresses human cGAS-HA; Puromycin selection markerDepositorInsertcGAS
TagsHAExpressionMammalianPromoterCMVAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_sfCherry3V(1-10)
Plasmid#117483PurposeLentiviral vector for constitutive expression of sfCherry3V(1-10)DepositorInsertsfCherry3V(1-10)
UseLentiviralExpressionMammalianMutation6 substitutions (K45R, G52D, L83W, T106A, K182R, …PromoterSFFVAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTarget_CAST
Plasmid#127926PurposeTarget plasmid containing PSP1DepositorInsertPSP1 target (GGTT PAM)
Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
HPV16 E7 TCR
Plasmid#122728PurposeExpress HPV16 E7 TCRDepositorInsertHPV16 E7 TCR
UseRetroviralAvailable SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synP-FLEX-splitTVA-EGFP-B19G (AAV1)
Viral Prep#52473-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-synP-FLEX-splitTVA-EGFP-B19G (#52473). In addition to the viral particles, you will also receive purified pAAV-synP-FLEX-splitTVA-EGFP-B19G plasmid DNA. Helper virus for monosynaptic tracing with rabies virus. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFP (Cre-dependent)Available SinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-mCherry
Plasmid#114470PurposemCherry ControlDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertmCherry
UseAAVPromoterEf1aAvailable SinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLS-P2A-GFP-PGK-Puro
Plasmid#110869PurposeLentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGMutationD10A and NLS sequence at the N-terminusPromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-tdTomato-P2A-BlasR (LRT2B)
Plasmid#110854PurposeLentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markersDepositorInsertsgRNA scaffold with spacer
UseLentiviralMutationWTPromoterU6Available SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAC-tag-N
Plasmid#108078PurposeGateway compatible destination vectors containing StrepIII/HA/BioIDDepositorAvailable SinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
hMLKL-Venus
Plasmid#106078Purposeexpression in mammalian cellsDepositorInserthuman MLKL-Venus (MLKL Human)
UseRetroviral; Tet-on retrovirusTagsVenusExpressionMammalianPromoterDox-inducibleAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Guide-EFS-SpCas9-P2A-EGFP
Plasmid#99248Purposesingle-vector system with EGFP reporter for use in CROPseq knockout screensDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsEGFPExpressionMammalianPromoterEFSAvailable SinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN113 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26
Plasmid#86233PurposeTargeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Neomycine selection. Auxin-inducible degron system.DepositorInsertosTir1
UseMouse TargetingTagsV5 tagExpressionMammalianAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDMyc-TNPO1
Plasmid#86081PurposeA mammalian expression plasmid encoding Transportin-1 (TNPO1) with N-terminus double Myc-tag.DepositorAvailable SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only