161,601 results

1. Stargazin-mCherry-FRB

   Plasmid

   #172443

   Purpose

   Expression of plasma membrane protein Stargazin tagged with mCherry-FRB

   Depositor

   Stephen Royle

   Article

   Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   Cacng2 (Cacng2 Mouse)
   

   Use

   Tags

   mCherry-HA-FRB

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDG02583

   Plasmid

   #104129

   Purpose

   Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   NEDD8-specific protease 1 (NEDP1)
   

   Use

   Tags

   14x Histidine tag, Maltose binding protein, and S…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGM1190

   Plasmid

   #69994

   Purpose

   OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces

   Depositor

   Günther Muth

   Article

   Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

   Inserts

   aac
   tsr
   rep
   sso
   TipA promoter
   dso
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiCRISPR-RFP657

   Plasmid

   #75162

   Purpose

   Derived from LentiCRISPR v1 but expresses RFP657 instead of puromycin resistance

   Depositor

   Beat Bornhauser

   Article

   McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   RFP657

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. AnkyrinB-2XHA

   Plasmid

   #31057

   Depositor

   Vann Bennett

   Article

   Ayalon et al J Biol Chem. 2011 Mar 4. 286(9):7370-8.

   Insert

   Ankyrin B (ANK2 Human)
   

   Use

   Tags

   2XHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pdsRED-Mono-N1-FoxO1

   Plasmid

   #34678

   Depositor

   Domenico Accili

   Insert

   FoxO1-RFP (Foxo1 Mouse)
   

   Use

   Tags

   Flag and RFP

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2012

   Availability

   Academic Institutions and Nonprofits only

7. AAV-hSyn-RiboL1-jGCaMP8f

   Plasmid

   #169249

   Purpose

   AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8f protein.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   RiboL1-jGCaMP8f
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Dec. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1-DRGFP

   Plasmid

   #113193

   Purpose

   DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.

   Depositor

   Adam Karpf

   Insert

   DR-GFP
   

   Use

   Donor construct with t2a-hygromycin for gene trap…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV GG hUbC-dsRED

   Plasmid

   #84034

   Purpose

   Contains Golden Gate cassette to express up to four gRNAs and dsRED

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDF-GFPplus

    Plasmid

    #172718

    Purpose

    Expression of a fast folding GFP variant GFP plus

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    GFP plus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. STIM1-EGFP

    Plasmid

    #210370

    Purpose

    Expresses STIM1 fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tet-inducible mCherry reporter

    Plasmid

    #64128

    Purpose

    Photoactivatable transcription system. mCherry reporter under the control of a tet-inducible promoter

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetOx13-CMV

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCJR01

    Plasmid

    #187888

    Purpose

    Escherichia coli plasmid with cloned regions of homology to Campylobacter jejuni chromosome flanking a NotI restriction site. pCJR01 functions as a suicide vector for integrating cloned NotI DNA.

    Depositor

    Gaylen Uhlich

    Article

    Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144

    Insert

    DNA insert has homology to a rRNA region of the Campylobacter jejuni chromosome with an added central NotI restriction site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVpuro-CMV-N-mCherry

    Plasmid

    #123221

    Purpose

    N-terminal mCherry tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. human beta-catenin pcDNA3

    Plasmid

    #16828

    Purpose

    Mammalian expression of human beta-catenin with Flag tag

    Depositor

    Eric Fearon

    Article

    Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFETCh_Donor (EMM0021)

    Plasmid

    #63934

    Purpose

    3X Flag with P2A-Neo for 3' tagging of endogenous proteins

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    Glycine-Serine-3XFlag-P2A-NeoR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-45b(+)-Tn5

    Plasmid

    #112112

    Purpose

    Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

    Depositor

    Frank Pugh

    Article

    Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

    Insert

    Tn5
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E54K E110K P242A L372P

    Promoter

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE2-TetOminiCMV-SV40LT

    Plasmid

    #136616

    Purpose

    Dox-inducible lentiviral vector expressing SV40 large T antigen for immortalizing cells

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    SV40 large T antigen
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETDuet S2

    Plasmid

    #171921

    Purpose

    Express RNA-based (p)ppGpp sensor and a NirFP

    Depositor

    Mingxu You

    Article

    Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

    Inserts

    RNA-based (p)ppGpp sensor
    NirFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLminP_Luc2P_RE4

    Plasmid

    #90338

    Purpose

    NRF2 - Antioxidant response element gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    NRF2

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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