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Addgene

hα1Ga-pDsRed
(Plasmid #45811)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45811 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDsRed-Express-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 11662
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hα1Ga
  • Alt name
    CACNA1G
  • Alt name
    Cav3.1a
  • Alt name
    calcium channel, voltage-dependent, T type, alpha 1G subunit
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6962
  • GenBank ID
    AF190860
  • Entrez Gene
    CACNA1G (a.k.a. Ca(V)T.1, Cav3.1, NBR13, SCA42, SCA42ND)
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Cav3.1 insert retains its STOP codon, so there is no expression of the Ds-Red. The 5'UTR is gone and contains a new SalI site. The 3' end of insert ends in the endogenous Kpn1 site.
Identification of Cav3.1 is described in Nature. 1998 Feb 26;391(6670):896-900.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hα1Ga-pDsRed was a gift from Edward Perez-Reyes (Addgene plasmid # 45811 ; http://n2t.net/addgene:45811 ; RRID:Addgene_45811)

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