-
Depositing Lab
-
Depositing OrganizationUniversity of Virginia
Located in United States of America -
Publication
-
Citations
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45812 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 45812-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 45812-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepcDNA3-HE3
-
Backbone manufacturerPerez-Reyes
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 12700
-
Modifications to backbonepcDNA3 has been modified to contain 54 bp of the 5 untranslated region of Xenopus beta globin. Previous studies have shown that this sequence can significantly enhance expression in Xenopus oocytes (Liman et al., 1992), and does not interfere with expression in mammalian cells (Lee et al., 1999).
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameα1Ga
-
Alt nameCACNA1G
-
Alt nameCav3.1
-
Alt namecalcium channel, voltage-dependent, T type, alpha 1G subunit
-
SpeciesR. norvegicus (rat)
-
Insert Size (bp)7200
-
GenBank IDAF027984
-
Entrez GeneCacna1g
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (destroyed during cloning)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer CMV-F; T7
- 3′ sequencing primer BGHrev
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Identification of Cav3.1 is described in Nature. 1998 Feb 26;391(6670):896-900. Note that there are some differences compared to GenBank AF027984 (H1061L, deletion of amino acid L at 1700, H1735D, F2149S and F2170S) which was sequenced manually at the time. The Addgene NGS sequence is accurate.
DNA (Catalog # 45812-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 45812-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
α1GA-HE3-pcDNA3 was a gift from Edward Perez-Reyes (Addgene plasmid # 45812 ; http://n2t.net/addgene:45812 ; RRID:Addgene_45812)
Map uploaded by the depositor.
