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(Plasmid #45812)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45812 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 12700
  • Modifications to backbone
    pcDNA3 has been modified to contain 54 bp of the 5 untranslated region of Xenopus beta globin. Previous studies have shown that this sequence can significantly enhance expression in Xenopus oocytes (Liman et al., 1992), and does not interfere with expression in mammalian cells (Lee et al., 1999).
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
  • Alt name
  • Alt name
    calcium channel, voltage-dependent, T type, alpha 1G subunit
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (destroyed during cloning)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer CMV-F; T7
  • 3′ sequencing primer BGHrev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Identification of Cav3.1 is described in Nature. 1998 Feb 26;391(6670):896-900. Note that there are some differences compared to GenBank AF027984 (H1061L, deletion of amino acid L at 1700, H1735D, F2149S and F2170S) which was sequenced manually at the time. The Addgene NGS sequence is accurate.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    α1GA-HE3-pcDNA3 was a gift from Edward Perez-Reyes (Addgene plasmid # 45812 ; ; RRID:Addgene_45812)