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a1Ic-HE3-pcDNA3
(Plasmid #45810)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45810 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 45810-D50 50 μg of DNA in Tris buffer $495
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DNA 45810-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA3-HE3
  • Backbone manufacturer
    Perez-Reyes
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 13200
  • Modifications to backbone
    pcDNA3 has been modified to contain 54 bp of the 5 untranslated region of Xenopus beta globin. Previous studies have shown that this sequence can significantly enhance expression in Xenopus oocytes (Liman et al., 1992), and does not interfere with expression in mammalian cells (Lee et al., 1999).
  • Vector type
    Mammalian Expression ; making cRNA for oocyte injections
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CACNA1I
  • Alt name
    Cav3.3c
  • Alt name
    calcium channel, voltage-dependent, T type, alpha 1I subunit
  • Alt name
    LT9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7700
  • GenBank ID
    AF393329
  • Entrez Gene
    CACNA1I (a.k.a. Cav3.3, ca(v)3.3)
  • Promoter CMV

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

coding region ends at 6568

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    a1Ic-HE3-pcDNA3 was a gift from Edward Perez-Reyes (Addgene plasmid # 45810 ; http://n2t.net/addgene:45810 ; RRID:Addgene_45810)

  • For your References section:

    Cloning and expression of the human T-type channel Ca(v)3.3: insights into prepulse facilitation. Gomora JC, Murbartian J, Arias JM, Lee JH, Perez-Reyes E. Biophys J. 2002 Jul;83(1):229-41. 10.1016/S0006-3495(02)75164-3 PubMed 12080115
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