|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45810||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 13200
Modifications to backbonepcDNA3 has been modified to contain 54 bp of the 5 untranslated region of Xenopus beta globin. Previous studies have shown that this sequence can significantly enhance expression in Xenopus oocytes (Liman et al., 1992), and does not interfere with expression in mammalian cells (Lee et al., 1999).
Vector typeMammalian Expression ; making cRNA for oocyte injections
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Alt namecalcium channel, voltage-dependent, T type, alpha 1I subunit
SpeciesH. sapiens (human)
Insert Size (bp)7700
Entrez GeneCACNA1I (a.k.a. Cav3.3, ca(v)3.3)
- Promoter CMV
- Cloning method Unknown
- 5′ sequencing primer CMV-F; T7
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
coding region ends at 6568
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:a1Ic-HE3-pcDNA3 was a gift from Edward Perez-Reyes (Addgene plasmid # 45810 ; http://n2t.net/addgene:45810 ; RRID:Addgene_45810)
For your References section:Cloning and expression of the human T-type channel Ca(v)3.3: insights into prepulse facilitation. Gomora JC, Murbartian J, Arias JM, Lee JH, Perez-Reyes E. Biophys J. 2002 Jul;83(1):229-41. 10.1016/S0006-3495(02)75164-3 PubMed 12080115