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pT3-EF1a-NICD1
(Plasmid #46047)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46047 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Notch1 (C-term)
  • Alt name
    Notch 1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2385
  • Mutation
    Cytoplasmic domain initiating at amino acid 1753
  • GenBank ID
    NM_008714.3
  • Entrez Gene
    Notch1 (a.k.a. 9930111A19Rik, Mis6, N1, Tan1, lin-12)
  • Promoter EF1a
  • Tag / Fusion Protein
    • Myc tag (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Notch 1 from Addgene plasmid 15131: pLIA mNIC myc.
  • Terms and Licenses

Depositor Comments

This plasmid is in the pT3-EF1a vector which contains loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid cannot not used with Cre for in vivo studies.

For in vivo use in mice with Cre, please use plasmid 86500.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT3-EF1a-NICD1 was a gift from Xin Chen (Addgene plasmid # 46047 ; http://n2t.net/addgene:46047 ; RRID:Addgene_46047)
  • For your References section:

    Cholangiocarcinomas can originate from hepatocytes in mice. Fan B, Malato Y, Calvisi DF, Naqvi S, Razumilava N, Ribback S, Gores GJ, Dombrowski F, Evert M, Chen X, Willenbring H. J Clin Invest. 2012 Aug 1;122(8):2911-5. doi: 10.1172/JCI63212. Epub 2012 Jul 17. 10.1172/JCI63212 PubMed 22797301