pT3-EF1a-YapS127A
(Plasmid #46049)

• Depositing Lab: Xin Chen
• Publication: Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1542.e12. doi: 10.1053/j.gastro.2013.02.009. Epub 2013 Feb 16.

Backbone

• Vector backbone: pT3-EF1a
• Backbone size w/o insert (bp): 7000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Yes-kinase associated protein
• Alt name: YAP2, YAP65
• Alt name: YAP2 isoform
• Species: H. sapiens (human)
• Insert Size (bp): 1467
• Mutation: S127A
• GenBank ID: NM_006106
• Entrez Gene: YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
• Promoter: EF1a
• Tag / Fusion Protein:
  • Flag tag (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Yap2-S127A from Addgene plasmid 19050: p2xFlag CMV2-YAP2-S127A
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is in the pT3-EF1a vector which contains loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid cannot not used with Cre for in vivo studies.