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pCR3.1-mIL2

Plasmid

#74467

Purpose

expression of mouse IL2 in mammalian cells

Depositor

Loree Heller

Article

Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

Insert

IL2 (Il2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCR3.1-mIL2

Plasmid

#74467

Purpose

expression of mouse IL2 in mammalian cells

Depositor

Loree Heller

Article

Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

Insert

IL2 (Il2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-MOG-Fc(DAPA)-AviTag-6xHis

Plasmid

#156506

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

MOG (MOG Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-MOG-Fc(DAPA)-AviTag-6xHis

Plasmid

#156506

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

MOG (MOG Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry- SEC61B

Plasmid

#121160

Purpose

Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

SEC61B (SEC61B )

Expression

Mammalian

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry- SEC61B

Plasmid

#121160

Purpose

Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

SEC61B (SEC61B )

Expression

Mammalian

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pSC101-Donor

Plasmid

#140630

Purpose

Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

LE, RE, KanR, temperature sensitive pSC101 origin of replication

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSC101-Donor

Plasmid

#140630

Purpose

Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

LE, RE, KanR, temperature sensitive pSC101 origin of replication

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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TFORF0096

Plasmid

#142459

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZEB2 (ZEB2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF0096

Plasmid

#142459

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZEB2 (ZEB2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-TID-HA3-KanMX6

Plasmid

#227980

Purpose

To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID

Tags

HA tag

Expression

Yeast

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-STAT1

Plasmid

#11987

Depositor

Steven Johnson

Insert

Signal transducer and activator of transcription 1 (STAT1 Human)

Tags

GFP

Expression

Mammalian

Mutation

A188T, Q718R

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-STAT1

Plasmid

#11987

Depositor

Steven Johnson

Insert

Signal transducer and activator of transcription 1 (STAT1 Human)

Tags

GFP

Expression

Mammalian

Mutation

A188T, Q718R

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-YY1

Plasmid

#169018

Purpose

Expression of YY1 driven by CMV promoter

Depositor

Claes Wadelius

Article

Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

Insert

human YY1 (YY1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-YY1

Plasmid

#169018

Purpose

Expression of YY1 driven by CMV promoter

Depositor

Claes Wadelius

Article

Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

Insert

human YY1 (YY1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-H3-23

Plasmid

#55058

Purpose

Localization: Nucleus/Histones, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

H3 (H3C2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-H3-23

Plasmid

#55058

Purpose

Localization: Nucleus/Histones, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

H3 (H3C2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Blast DEST (w524-1)

Plasmid

#19065

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Blast DEST (w524-1)

Plasmid

#19065

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-FOXG1

Plasmid

#153124

Purpose

Expression of Flag tagged human FOXG1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXG1 (FOXG1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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