pcDNA3 Myc2- Hs TTP WT
(Plasmid #107008)

• Purpose: Expresses epitope-tagged wild-type human TTP
• Depositing Lab: Julian Downward
• Publication: Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6. doi: 10.1016/j.immuni.2017.11.016. Epub 2017 Dec 12.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Thermo Scientific
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: tristetraprolin
• Alt name: TTP, ZFP36
• Species: H. sapiens (human)
• Insert Size (bp): 1897
• GenBank ID:
• Entrez Gene: ZFP36 (a.k.a. G0S24, GOS24, NUP475, RNF162A, TIS11, TTP, zfp-36)
• Tag / Fusion Protein:
  • Double Myc peptide epitope tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: pcDNA3.1 FP: CTCTGGCTAACTAGAGAAC
• 3′ sequencing primer: pcDNA3.1 RP/1: CAAACAACAGATGGCTGGC

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry