Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||23001||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Mutationvaline inserted at aa#2
Entrez GeneKHSRP (a.k.a. FBP2, FUBP2, KSRP)
/ Fusion Proteins
- EGFP (N terminal on insert)
- His (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
The KHSRP insert in this plasmid contains an extra amino acid, valine, at position #2.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEGFPC1-6XHis-FLKSRP was a gift from Douglas Black (Addgene plasmid # 23001 ; http://n2t.net/addgene:23001 ; RRID:Addgene_23001)
For your References section:Differentiation-induced colocalization of the KH-type splicing regulatory protein with polypyrimidine tract binding protein and the c-src pre-mRNA. Hall MP, Huang S, Black DL. Mol Biol Cell. 2004 Feb . 15(2):774-86. 10.1091/mbc.e03-09-0692 PubMed 14657238