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Genetic switches designed for eukaryotic cells and controlled by serine integrases.
Gomide MS, Sales TT, Barros LRC, Limia CG, de Oliveira MA, Florentino LH, Barros LMG, Robledo ML, Jose GPC, Almeida MSM, Lima RN, Rehen SK, Lacorte C, Melo EO, Murad AM, Bonamino MH, Coelho CM, Rech E
Commun Biol. 2020 May 22;3(1):255. doi: 10.1038/s42003-020-0971-8.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
127504INCTbiosyn-pEF-GFP(rc)2Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 2 attachment sites.
127505INCTbiosyn-pEF-GFP(rc)4Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 4 attachment sites.
127506INCTbiosyn-pEF-GFP(rc)5Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 5 attachment sites.
127507INCTbiosyn-pEF-GFP(rc)7Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 7 attachment sites.
127508INCTbiosyn-pEF-GFP(rc)9Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 9 attachment sites.
127509INCTbiosyn-pEF-GFP(rc)13Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 13 attachment sites.
127510INCTbiosyn-pEF-GFP(rc)phiC31Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase phiC31 attachment sites.
127511INCTbiosyn-pEF-GFP(rc)Bxb1Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase Bxb1 attachment sites.
127512INCTbiosyn-pUB-HspINT2Plasmid encodes H. sapiens codon optimized Integrase 2.
127513INCTbiosyn-pUB-HspINT4Plasmid encodes H. sapiens codon optimized Integrase 4.
127514INCTbiosyn-pUB-HspINT5Plasmid encodes H. sapiens codon optimized Integrase 5.
127515INCTbiosyn-pUB-HspINT7Plasmid encodes H. sapiens codon optimized Integrase 7.
127516INCTbiosyn-pUB-HspINT9Plasmid encodes H. sapiens codon optimized Integrase 9.
127517INCTbiosyn-pUB-HspINT13Plasmid encodes H. sapiens codon optimized Integrase 13.
127518INCTbiosyn-pUB-HspINTphiC31Plasmid encodes H. sapiens codon optimized Integrase phiC31.
127519INCTbiosyn-pUB-HspINTBxb1Plasmid encodes H. sapiens codon optimized Integrase Bxb1.
127520INCTbiosyn-p35S(rc)2_4_5-GFPPlasmid has an inverted CaMV 35S promoter sequence (reverse complement) flanked by attB and attP attachment sites of integrases 2, 4, and 5. EGFP coding sequence is in the forward orientation.
127521INCTbiosyn-p35S-GFP(rc)2Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 2 attachment sites.
127522INCTbiosyn-p35S-GFP(rc)4Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 4 attachment sites.
127523INCTbiosyn-p35S-GFP(rc)5Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 5 attachment sites.
127524INCTbiosyn-p35S-GFP(rc)7Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 7 attachment sites.
127525INCTbiosyn-p35S-GFP(rc)9Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 9 attachment sites.
127526INCTbiosyn-p35S-GFP(rc)13Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 13 attachment sites.
127527INCTbiosyn-p35S-GFP(rc)phiC31Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase phiC31 attachment sites.
127528INCTbiosyn-p35S-GFP(rc)Bxb1Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase Bxb1 attachment sites.
127529INCTbiosyn-pAct-AtINT2Plasmid encodes A. thaliana codon optimized Integrase 2.
127530INCTbiosyn-pAct-AtINT4Plasmid encodes A. thaliana codon optimized Integrase 4.
127531INCTbiosyn-pAct-AtINT5Plasmid encodes A. thaliana codon optimized Integrase 5.
127532INCTbiosyn-pAct-AtINT7Plasmid encodes A. thaliana codon optimized Integrase 7.
127533INCTbiosyn-pAct-AtINT9Plasmid encodes A. thaliana codon optimized Integrase 9.
127534INCTbiosyn-pAct-AtINT13Plasmid encodes A. thaliana codon optimized Integrase 13.
127535INCTbiosyn-pAct-AtINTphiC31Plasmid encodes A. thaliana codon optimized Integrase phiC31.
127536INCTbiosyn-pAct-AtINTBxb1Plasmid encodes A. thaliana codon optimized Integrase Bxb1.

Antibodies from Article