INCTbiosyn-p35S-GFP(rc)2
(Plasmid #127521)

• Purpose: Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 2 attachment sites.
• Depositing Lab: Martin Bonamino
• Publication: Gomide et al Commun Biol. 2020 May 22;3(1):255. doi: 10.1038/s42003-020-0971-8.

Backbone

• Vector backbone: pUC18
• Vector type: Plant Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP reverse complement coding sequence flanked by attB/attP Integrase 2 attachment sites.
• Alt name: Switch vector INT 2
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1531

Cloning Information

• Cloning method: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The CaMV 35S promoter sequence is derived from iGEM registry (BBa_K1547006); the EGFP coding sequence from Chiu, W. et al. Curr. Biol. 6, 325-330 (1996); the attB/P integrase 2 attachment sites sequences from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014); and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991).