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Addgene

pCGN-HCF-1 fl
(Plasmid #53309)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53309 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCGN
  • Backbone size w/o insert (bp) 5063
  • Total vector size (bp) 11252
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HCFC1
  • Alt name
    HCF-1
  • Alt name
    HCF-1 fl
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6105
  • GenBank ID
    L20010.1
  • Entrez Gene
    HCFC1 (a.k.a. CFF, HCF, HCF-1, HCF1, HFC1, MAHCX, MRX3, PPP1R89, VCAF, XLID3)
  • Promoter CMV
  • Tags / Fusion Proteins
    • HA (N terminal on backbone)
    • c-myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV_for
  • 3′ sequencing primer -
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCGN-HCF-1 fl was a gift from Winship Herr (Addgene plasmid # 53309 ; http://n2t.net/addgene:53309 ; RRID:Addgene_53309)
  • For your References section:

    The VP16 accessory protein HCF is a family of polypeptides processed from a large precursor protein. Wilson AC, LaMarco K, Peterson MG, Herr W. Cell. 1993 Jul 16;74(1):115-25. 10.1016/0092-8674(93)90299-6 PubMed 8392914