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pcDNA5FRT/Flag-OGT1
(Plasmid #29760)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 29760 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA5FRT
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OGT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3141
  • Mutation
    N764D and K1010E
  • GenBank ID
    NP_858059.1 NM_181672
  • Entrez Gene
    OGT (a.k.a. HINCUT-1, HRNT1, MRX106, O-GLCNAC, OGT1)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

The OGT1 insert contains the following mutations N764D and K1010E. These were present in the plasmid as it was described in the publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5FRT/Flag-OGT1 was a gift from Joan Conaway (Addgene plasmid # 29760 ; http://n2t.net/addgene:29760 ; RRID:Addgene_29760)
  • For your References section:

    Subunit composition and substrate specificity of a MOF-containing histone acetyltransferase distinct from the male-specific lethal (MSL) complex. Cai Y, Jin J, Swanson SK, Cole MD, Choi SH, Florens L, Washburn MP, Conaway JW, Conaway RC. J Biol Chem. 2010 Feb 12. 285(7):4268-72. 10.1074/jbc.C109.087981 PubMed 20018852