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pBJG1 T7-8xHis-OGT
(Plasmid #154271)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154271 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 8412
  • Modifications to backbone
    subcloned MCS from alternate pET vector to take tag as described in paper SI
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    O-GlcNAc Transferase
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    bacterial codon optimized
  • Tags / Fusion Proteins
    • T7 leader (N terminal on insert)
    • 8x His (N terminal on insert)
    • HRV3C protease site (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBJG1 T7-8xHis-OGT was a gift from Suzanne Walker (Addgene plasmid # 154271 ; ; RRID:Addgene_154271)
  • For your References section:

    Discovery of O-GlcNAc transferase inhibitors. Gross BJ, Kraybill BC, Walker S. J Am Chem Soc. 2005 Oct 26;127(42):14588-9. doi: 10.1021/ja0555217. 10.1021/ja0555217 PubMed 16231908