pscAAV-GFP
(Plasmid #32396)

• Depositing Lab: John T Gray
• Publication: Gray et al Methods Mol Biol. 2011;807:25-46.

Backbone

• Vector backbone: pSub201
• Backbone size w/o insert (bp): 8310
• Vector type: Mammalian Expression, AAV ; Adeno-Associated Virus

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: Users must confirm the absence of deletions (recombinations) in this vector by diagnostic digests. (1) An AhdI digest should produce the following pattern of bands: 1557 bp, 1478 bp and 1334 bp. (2) An SrfI digest should produce the following pattern of bands: 2801 bp and 1546 bp. Please consult the associated publication listed below for an example gel image. *See Addgene note below regarding the band sizes.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GFP
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Multiple (see map) (not destroyed)
• 3′ cloning site: Multiple (see map) (not destroyed)
• 5′ sequencing primer: TGGCACCAAAATCAACGGGACTTTCCAAAATGT
• 3′ sequencing primer: AAAACCTCCCACACCTCCCCCTGAAC

Resource Information

• Supplemental Documents:
  • LaserGene SeqBuilder file for pscAAV-GFP
• Articles Citing this Plasmid:
  • 22 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene detected a 22 bp deletion which may affect packaging efficiency but does not affect the overall plasmid function. Please screen multiple colonies as noted by the depositor in the growth instructions. The exact number of bases is different compared to the Addgene verified sequence, however they are approximately the same size.