ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons.
Willems J, de Jong APH, Scheefhals N, Mertens E, Catsburg LAE, Poorthuis RB, de Winter F, Verhaagen J, Meye FJ, MacGillavry HD
PLoS Biol. 2020 Apr 10;18(4):e3000665. doi: 10.1371/journal.pbio.3000665. PubMed Article
Willems J, de Jong APH, Scheefhals N, Mertens E, Catsburg LAE, Poorthuis RB, de Winter F, Verhaagen J, Meye FJ, MacGillavry HD
PLoS Biol. 2020 Apr 10;18(4):e3000665. doi: 10.1371/journal.pbio.3000665. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 131471 | pORANGE Cloning template vector | Cloning template form ORANGE method based knock-in constructs |
| 131472 | pORANGE C9orf4-GFP KI | Endogenous tagging of C9orf4: C-terminal (amino acid position: STOP codon) |
| 131473 | pORANGE CACNG8-GFP KI #1 | Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: V376) |
| 131474 | pORANGE CACNG8-GFP KI #2 | Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: A408) |
| 131475 | pORANGE Cplx1-GFP KI | Endogenous tagging of Complexin1: C-terminal (amino acid position: L128) |
| 131476 | pORANGE Cplx2-GFP KI | Endogenous tagging of Complexin2: C-terminal (amino acid position: L128) |
| 131477 | pORANGE Dlg4-GFP KI | Endogenous tagging of PSD95: C-terminal (amino acid position: R721) |
| 131478 | pORANGE Doc2A-GFP KI | Endogenous tagging of Doc2a: C-terminal (amino acid position: L402) |
| 131479 | pORANGE GFP-Actb KI | Endogenous tagging of βactin: N-terminal (amino acid position: D2) |
| 131480 | pORANGE GFP-CACNA1A KI | Endogenous tagging of CaV2.1, P/Q: N-terminal (amino acid position: G10) |
| 131481 | pORANGE GFP-CACNA1E KI | Endogenous tagging of CaV2.3, R: N-terminal (amino acid position: G5) |
| 131482 | pORANGE GFP-CADPS KI | Endogenous tagging of CAPS1: N-terminal (amino acid position: S39) |
| 131483 | pORANGE GFP-Clta KI | Endogenous tagging of Clathrin light chain α: N-terminal (amino acid position: M1) |
| 131484 | pORANGE GFP-Camk2a KI | Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon) |
| 131485 | pORANGE GFP-Grin1 KI | Endogenous tagging of GluN1: N-terminal (amino acid position: A20) |
| 131486 | pORANGE GFP-Grin2a KI | Endogenous tagging of GluN2a: N-terminal (amino acid position: A25) |
| 131487 | pORANGE GFP-Grin2b KI | Endogenous tagging of GluN2b: N-terminal (amino acid position: S34) |
| 131488 | pORANGE GFP-Syt7 KI | Endogenous tagging of Synaptotagmin-7: N-terminal (amino acid position: P5) |
| 131489 | pORANGE Gria1-GFP KI | Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon) |
| 131490 | pORANGE Gria2-GFP KI | Endogenous tagging of GluA2: C-terminal (amino acid position: STOP codon) |
| 131491 | pORANGE Gria3-GFP KI | Endogenous tagging of GluA3: C-terminal (amino acid position: STOP codon) |
| 131492 | pORANGE GSG1l-GFP KI | Endogenous tagging of GSG1-l: C-terminal (amino acid position: STOP codon) |
| 131493 | pORANGE mEos3.2-Camk2a KI | Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon) |
| 131494 | pORANGE Rims1-GFP KI | Endogenous tagging of RIM1: C-terminal (amino acid position: S1615) |
| 131495 | pORANGE Rims2-GFP KI | Endogenous tagging of RIM2: C-terminal (amino acid position: S1551) |
| 131496 | pORANGE Shank2-GFP KI | Endogenous tagging of Shank2: C-terminal (amino acid position: STOP codon) |
| 131497 | pORANGE Tubb3-GFP KI | Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon) |
| 131498 | pORANGE unc13a GFP KI | Endogenous tagging of Munc13-1: C-terminal (amino acid position: A1762) |
| 131499 | pORANGE GFP-RAB11a KI | Endogenous tagging of RAB11: N-terminal (amino acid position: before startcodon) |
| 131500 | pORANGE Shank1-GFP KI | Endogenous tagging of Shank1: C-terminal (amino acid position: STOP codon) |
| 131501 | pORANGE GFP-Nlgn3 KI | Endogenous tagging of Neuroligin-3: N-terminal (amino acid position: T37) |
| 131502 | pORANGE WASF1-GFP KI | Endogenous tagging of WASP1/WAVE1: C-terminal (amino acid position: STOP codon) |
| 131503 | pORANGE GFP-Arpc5 KI | Endogenous tagging of Arp2/3 complex subunit 5: C-terminal (amino acid position: STOP codon) |
| 131504 | pORANGE CACNG2-GFP KI | Endogenous tagging of Tarpγ2/Stargazin: Intramolecular (amino acid position: S265) |
| 131505 | pFUGW mCherry-KASH | mCherry_KASH epression under hUBC promotor; serves as backbone for ORANGE lenti knock-in |
| 131506 | pFUGW SpCas9 | lentiviral expression of SpCas9 under hUBC promotor |
| 131507 | pFUGW ORANGE Gria1-GFP KI _ mCherry-KASH | Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon) |
| 131508 | pFUGW ORANGE Tubb3-GFP KI _ mCherry-KASH | Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon) |
| 139651 | pORANGE LOX Cloning template vector | Cloning template for ORANGE-CAKE conditional knock-in constructs |
| 139652 | pFSW FLEx mCherry-KASH | Conditional expression of mCherry-KASH |
| 139653 | pFSW FLEx Synapsin-FLAG | Conditional expression of Synapsin-FLAG |
| 139654 | pAAV MCS mCherry-KASH | Cloning template for AAV-based ORANGE knock-in constructs |
| 139655 | pAAV ORANGE Gria1-HaloTag KI | Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon) |
| 139656 | pAAV ORANGE Dlg4-HaloTag KI | Endogenous tagging of PSD95: C-terminal (amino acid position: R721) |
| 139657 | pORANGE GFP-Pclo KI | Endogenous tagging of Piccolo: N-terminal (amino acid position: G2) |
| 139658 | pORANGE GFP-Grin1 KI #2 | Endogenous tagging of GluN1: N-terminal (amino acid position: D23) |
| 139659 | pORANGE GFP-Grin1 KI #3 | Endogenous tagging of GluN1: N-terminal (amino acid position: A20) |
| 139660 | pORANGE GFP-Cacnb1 KI | Endogenous tagging of CaVβ1: N-terminal (amino acid position: S8) |
| 139661 | pORANGE Cacnb2-GFP KI | Endogenous tagging of CaVβ2: C-terminal (amino acid position: Q655) |
| 139662 | pORANGE Cacnb3-GFP KI | Endogenous tagging of CaVβ3: C-terminal (amino acid position: W479) |
| 139663 | pORANGE Cacnb4-GFP KI | Endogenous tagging of CaVβ4: C-terminal (amino acid position: H417) |
| 139664 | pORANGE GFP-Bsn KI | Endogenous tagging of Bassoon: N-terminal (amino acid position: L7) |
| 139665 | pORANGE Bsn-GFP KI | Endogenous tagging of Bassoon: C-terminal (amino acid position: F3938) |
| 139666 | pORANGE GFP-Actb KI #2 | Endogenous tagging of β-actin: N-terminal (amino acid position: before startcodon) |