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iATPSnFR2: A high-dynamic-range fluorescent sensor for monitoring intracellular ATP.

Marvin JS, Kokotos AC, Kumar M, Pulido C, Tkachuk AN, Yao JS, Brown TA, Ryan TA
Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2314604121. doi: 10.1073/pnas.2314604121. Epub 2024 May 15. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
209651pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTagExpression of iATPSnFR2, high affinity
209652pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTagExpression of iATPSnFR2, medium affinity
209653pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTagExpression of iATPSnFR2, low affinity
209654pAAV.CAG.(cyto).cpSFGFP.HaloTagNon-responsive control
209655pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTagExpression of iATPSnFR2, high affinity
209656pAAV.CAGFLEX.(cyto).iATPSnFR2.A95K.HaloTagExpression of iATPSnFR2, medium affinity
209657pAAV.CAGFLEX.(cyto).iATPSnFR2.A95A.A119L.HaloTagExpression of iATPSnFR2, low affinity
209658pAAV.CAGFLEX.(cyto).cpSFGFP.HaloTagNon-responsive control
209659pAAV.GFAP.(cyto).iATPSnFR2.S29W.A95K.HaloTagExpression of iATPSnFR2, high affinity
209660pAAV.GFAP.(cyto).iATPSnFR2.A95K.HaloTagExpression of iATPSnFR2, medium affinity
209661pAAV.GFAP.(cyto).iATPSnFR2.A95A.A119L.HaloTagExpression of iATPSnFR2, low affinity
209662pAAV.GFAP.(cyto).cpSFGFP.HaloTagNon-responsive control
209663pAAV.hSyanpsin.(cyto).iATPSnFR2.S29W.A95K.HaloTagExpression of iATPSnFR2, high affinity
209664pAAV.hSynapsin.(cyto).iATPSnFR2.A95K.HaloTagExpression of iATPSnFR2, medium affinity
209665pAAV.hSynapsin.(cyto).iATPSnFR2.A95A.A119L.HaloTagExpression of iATPSnFR2, low affinity
209666pAAV.hSynapsin.(cyto).cpSFGFP.HaloTagNon-responsive control
209722pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.HaloTagExpresses mitochondrially targeted low affinity ratiometric ATP sensor
209723pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.miRFP670nano3Expresses mitochondrially targeted low affinity ratiometric ATP sensor
209724plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.HaloTagExpresses cytosolic low affinity ratiometric ATP sensor
209725plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3Expresses cytosolic low affinity ratiometric ATP sensor
209726plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTagExpresses cytosolic medium affinity ratiometric ATP sensor
209727plenti.CamKII.(cyto).iATPSnFR2.A95K.miRFP670nano3Expresses cytosolic medium affinity ratiometric ATP sensor
209729plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.HaloTagExpresses presynaptic low affinity ratiometric ATP sensor
209730plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3Expresses presynaptic low affinity ratiometric ATP sensor
214925pAAV.CAG.(mito).cpSFGFP.HaloTagExpresses mitochondrially targeted non-responsive control
214926plenti.CamKII.(synapto).cpsfGFP.miRFP670nano3Expresses presynaptic non-responsive control

Antibodies from Article