pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.HaloTag
(Plasmid
#209722)
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PurposeExpresses mitochondrially targeted low affinity ratiometric ATP sensor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209722 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV.CAG
- Total vector size (bp) 7201
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name(mito).iATPSnFR2.A95A.A119L.HaloTag
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Insert Size (bp)2490
- Promoter CAG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer CAG-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.08.24.554624v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.HaloTag was a gift from Timothy Ryan (Addgene plasmid # 209722 ; http://n2t.net/addgene:209722 ; RRID:Addgene_209722) -
For your References section:
iATPSnFR2: A high-dynamic-range fluorescent sensor for monitoring intracellular ATP. Marvin JS, Kokotos AC, Kumar M, Pulido C, Tkachuk AN, Yao JS, Brown TA, Ryan TA. Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2314604121. doi: 10.1073/pnas.2314604121. Epub 2024 May 15. 10.1073/pnas.2314604121 PubMed 38748581