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p201N Cas9
(Plasmid #59175)


Item Catalog # Description Quantity Price (USD)
Plasmid 59175 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6200
  • Total vector size (bp) 14349
  • Modifications to backbone
    Inclusion of aph gene for bacterial selection on kanamycin.
  • Vector type
  • Selectable markers
    G418, kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
  • Alt name
    Cas9 human-codon optimized
  • Species
  • Insert Size (bp)
  • Promoter 2x35S

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site I-SceI (not destroyed)
  • 3′ cloning site I-SceI (not destroyed)
  • 5′ sequencing primer ACATGCACCTAATTTCACTAGATGT
  • 3′ sequencing primer TGTAAAACGACGGCCAGT
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
  • Species
    E. coli
  • Insert Size (bp)
  • Promoter StUbi-3P

Cloning Information for Gene/Insert 2

  • Cloning method Unknown
  • 5′ sequencing primer AGCGGATAACAATTTCACACAGGA
  • 3′ sequencing primer GCAGAGCTTACACTCTCATTC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p201N Cas9 was a gift from Wayne Parrott (Addgene plasmid # 59175 ; ; RRID:Addgene_59175)
  • For your References section:

    Targeted genome modifications in soybean with CRISPR/Cas9. Jacobs TB, LaFayette PR, Schmitz RJ, Parrott WA. . BMC Biotechnology. 2015;15:16 10.1186/s12896-015-0131-2