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Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.
Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
75381pHAGE-TO-dCas9dCas9
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75383pHAGE-TO-MCP-3XBFPnlsMCP-3XBFPnls
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75384pHAGE-EFS-MCP-3XBFPnlsMCP-3XBFPnls
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75385pHAGE-EFS-PCP-3XGFPnlsPCP-3XGFPnls
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75386pHAGE-EFS-PCP-3XNeon-nlsPCP-3XNeon-nls
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75387pHAGE-EFS-N22p-3XRFPnlsN22p-3XRFPnls
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75388pLH-sgRNA1sgRNA1
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75389pLH-sgRNA1-2XMS2sgRNA1-2XMS2
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75390pLH-sgRNA1-2XPP7sgRNA1-2XPP7
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75391pLH-sgRNA1-2XboxBsgRNA1-2XboxB
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75392pLH-sgRNA1-MS2-PP7sgRNA1-MS2-PP7
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75393pLH-sgRNA1-PP7-boxBsgRNA1-PP7-boxB
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75396pLH-sgRNA1-boxB-MS2sgRNA1-boxB-MS2
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75397pLH-sgRNA1-boxB-MS2-PP7sgRNA1-boxB-MS2-PP7
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75398pCRISPRainbow-DONOR1CRISPRainbow-DONOR1
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