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Constitutive Gs activation using a single-construct tetracycline-inducible expression system in embryonic stem cells and mice.
Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR
Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
24414pEnt L1L3 tTA-2
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24415pEnt L1L3 EF1a-tTA-3
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24416pEnt R3L2 TetO(fl)-2
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24417pEnt R3L2 TetO(sh) mCh-Rs1-2
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24418pRosa26 R1-ccdB-R2 RexNeo PI-SceI
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24419Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2
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26797Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)
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26798pEnt L1L3-MCS
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26799pEnt R3L2-MCS
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26803pEnt L1L3 EF1a-tTA-2
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27105pEnt L1L3 tTA-3
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27106pEnt L1L3 rtTA-3
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27107pEnt R3L2 TetO(fl)-3
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29496pcDNA3.2 GW delCMV
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