pRosa26 R1-ccdB-R2 RexNeo PI-SceI
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24418||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerSoriano Lab (Addgene Plasmid# 21714)
- Backbone size w/o insert (bp) 9846
Vector typeMouse Targeting
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Growth instructionsccdB cells
Alt namepR26 R1R2 RexNeo PI-SceI
Insert Size (bp)3788
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Murine Rosa26 targeting vector with R1R2 Gateway sites. Includes a ccdB cassette, Rex-Neo selection marker cassette, and DTA selection cassette.
Plasmid was constructed as follows: The pRosa26-1 plasmid (Addgene plasmid number 21714) was digested at the XbaI cloning site and a PCR product containing the attR1-ccdb-attR2 sequences from pDest27 and an additional PacI site was introduced. The Rex-Neo resistance cassette from the αMHC-eGFP-Rex-Neo lentivirus plasmid was PCR-cloned into the PacI site to allow for antibiotic selection in eukaryotic cells with G418
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRosa26 R1-ccdB-R2 RexNeo PI-SceI was a gift from Edward Hsiao (Addgene plasmid # 24418 ; http://n2t.net/addgene:24418 ; RRID:Addgene_24418)
For your References section:Constitutive Gs activation using a single-construct tetracycline-inducible expression system in embryonic stem cells and mice. Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR. Stem Cell Res Ther. 2011 Mar 4. 2(2):11. 10.1186/scrt52 PubMed 21375737