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(Plasmid #24419)


Item Catalog # Description Quantity Price (USD)
Plasmid 24419 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Plasmid 21714: pROSA26-1 (Soriano Lab)
  • Backbone size w/o insert (bp) 4486
  • Vector type
    Mouse Targeting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Construct expresses both mCherry and Rs1 via a P2A ribosomal skip sequence
  • Entrez Gene
    HTR4 (a.k.a. 5-HT4, 5-HT4R)
  • Tags / Fusion Proteins
    • mCherry (N terminal on backbone)
    • FLAG (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Rosa26 targeting vector containing the EF1α-tTA/TetO-mCh-Rs1 cassette flanked by insulator sequences

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2 was a gift from Edward Hsiao (Addgene plasmid # 24419 ; ; RRID:Addgene_24419)
  • For your References section:

    Constitutive Gs activation using a single-construct tetracycline-inducible expression system in embryonic stem cells and mice. Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR. Stem Cell Res Ther. 2011 Mar 4. 2(2):11. 10.1186/scrt52 PubMed 21375737