pcDNA3.2 GW delCMV
(Plasmid #29496)

• Purpose: (Empty Backbone)
• Depositing Lab: Edward Hsiao
• Publication: Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Backbone

• Vector backbone: pcDNA3.2/V5-DEST
• Backbone manufacturer: Invitrogen
• Backbone size (bp): 7142
• Modifications to backbone: Based on Invitrogen pcDNA3.2/V5-DEST plasmid, with the majority of the CMV promoter removed by restriction digest with SpeI and SacI, filled in using Klenow fragment, and religated
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: 37C 24hours in LB-Amp100
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7 (across AttR1)

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Generic promoter-less destination vector