161,568 results

1. NES-Grx1-roGFP2

   Plasmid

   #236551

   Purpose

   Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the cytoplasm.

   Depositor

   Pablo Rivera-Fuentes

   Insert

   NES-Grx1-roGFP2 (NES Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. T/Brachyury-eGFP Rex Neo

   Plasmid

   #21222

   Depositor

   Mark Mercola

   Article

   Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

   Insert

   Brachyury promoter
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   July 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ133A

   Plasmid

   #69275

   Purpose

   Golden Gate entry vector; 3rd gRNA under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS1_Puro_hPGK1_EGFP_Donor

   Plasmid

   #178088

   Purpose

   HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human PGK1

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. human beta-catenin pcDNA3

   Plasmid

   #16828

   Purpose

   Mammalian expression of human beta-catenin with Flag tag

   Depositor

   Eric Fearon

   Article

   Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

   Insert

   beta-catenin (CTNNB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-mCherry-flex-dtA

   Plasmid

   #58536

   Purpose

   Expresses diphtheria toxin A subunit in Cre positive cells, and mCherry in all the infected cells

   Depositor

   Naoshige Uchida

   Article

   Wu et al Nature. 2014 May 15;509(7500):325-30. do

   Insert

   dtA (DIP_RS18250 )
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAG-BFP-sesRNA-tTA2-WPRE-pA

   Plasmid

   #192070

   Purpose

   Expression of BFP, sesRNA in mammalian cells, with tTA2 as efRNA.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for tdTom
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-AAV-syn-jGCaMP8f-WPRE

   Plasmid

   #162376

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   jGCaMP8f
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Q315L

   Promoter

   Synapsin

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. CAG-FR-GECO1c

   Plasmid

   #163682

   Purpose

   Far-red fluorescent calcium indicator FR-GECO1c

   Depositor

   Robert Campbell

   Article

   Dalangin et al bioRxiv 2020.11.12.380089

   Insert

   FR-GECO1c
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGM1192

    Plasmid

    #115683

    Purpose

    E. coli-Streptomyces shuttle vector for gene expression

    Depositor

    Günther Muth

    Insert

    mCherry, aac(3)IV, tsr
    

    Use

    Tags

    Strep-tagII on mCherry, codon optimized

    Expression

    Bacterial

    Mutation

    mCherry codon optimized for Streptomyces

    Promoter

    SF14*

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3-BoxB

    Plasmid

    #92004

    Purpose

    Expresses firefly luciferase with BoxB sequences in 3' UTR

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    Firefly Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGSHU

    Plasmid

    #72244

    Purpose

    Contains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoter

    Depositor

    Michael Resnick, Francesca Storici

    Article

    Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

    Inserts

    KlURA3
    hyg
    I-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLifeAct-mScarlet3_N1

    Plasmid

    #189767

    Purpose

    Construct for labelling actin filaments in mammalian cells with lifeact-mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    LifeAct-mScarlet3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKb-TnpB2

    Plasmid

    #215334

    Purpose

    ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site

    Depositor

    Kutubuddin Molla

    Article

    Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10

    Insert

    Rice codon optimized ISDra2 TnpB
    

    Use

    Tags

    Not applicable

    Expression

    Plant

    Mutation

    Promoter

    Rice ubiquitin promoter for TnpB and maize ubiqui…

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1-REST_sh_seq1_WPRE

    Plasmid

    #127573

    Purpose

    shRNA to knock down RE1-silencing transcription factor (REST)

    Depositor

    Malin Parmar

    Article

    Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

    Insert

    shRNA for knock down of REST (REST Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-ldhGFP

    Plasmid

    #27170

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    lactate dehydrogenase promoter region fused with modified green fluorescent protein 5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHEN2-SG4 R105A

    Plasmid

    #196072

    Purpose

    Express mutant (R105A) SG4 in E.coli

    Depositor

    Shankar Balasubramanian

    Article

    Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

    Insert

    SG4 R105A
    

    Use

    Tags

    3XFLAG and 6XHis

    Expression

    Bacterial

    Mutation

    R105A mutation in SG4

    Promoter

    T7

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 flag p53

    Plasmid

    #10838

    Depositor

    Thomas Roberts

    Article

    Gjoerup et al J Virol. 2001 Oct . 75(19):9142-55.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CMV-GFP-NMHC II-A

    Plasmid

    #11347

    Depositor

    Robert Adelstein

    Article

    Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

    Insert

    NMHC II-A (MYH9 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(-)-qmTQ2-cAMP

    Plasmid

    #225250

    Purpose

    mammalian expression of qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-cAMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge