168,515 results

1. pB80-hsKIF5B(1-560)Rigor-L-2xHA

   Plasmid

   #193718

   Purpose

   Expression of HA tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-560)Rigor-L-2xHA (KIF5B Human, Synthetic)
   

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-560), RIGOR: G234A

   Promoter

   Chicken beta-actin

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF2957

   Plasmid

   #144433

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   HNF4A (HNF4A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBtet-BP

   Plasmid

   #60496

   Purpose

   SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and puromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Expression

   Mammalian

   Promoter

   TCE & RPBSA

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV-EF1a-BFP-W

   Plasmid

   #208757

   Purpose

   Lentiviral vector expressing BFP

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. N-Cadherin-EGFP

   Plasmid

   #18870

   Depositor

   Valeri Vasioukhin

   Article

   Nechiporuk et al Dev Cell. 2007 Sep . 13(3):338-50.

   Insert

   N-Cadherin (Cdh2 Mouse)
   

   Tags

   EGFP, Kanamycin, and N-Cadherin

   Expression

   Mammalian

   Available Since

   Aug. 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEM705 Gs15

   Plasmid

   #109360

   Purpose

   GsX chimera with Gα15 C-terminus

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Gs15 chimera
   

   Tags

   None

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEGFP-N1-MST2

   Plasmid

   #166475

   Purpose

   Expresses fusion of mEGFP and MST2

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mEGFP, MST2 (STK3 Human)
   

   Expression

   Mammalian

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. U6-BbsI-DR_CMV-minidCas13X.1-REPAIRv2-BGHpA_CMV-EGFP-BGHpA

   Plasmid

   #171383

   Purpose

   Expression vector for encoding a human codon-optimized minidCas13X.1-REPAIRv2 driven by CMV promoter,EGFP driven by CMV promoter and U6-driven crRNAs cloning site.

   Depositor

   Hui Yang

   Article

   Xu et al Nat Methods. 2021 May;18(5):499-506. doi

   Insert

   humanized minidCas13X.1-REPAIRv2
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV, U6

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEasyG1_nat

   Plasmid

   #184911

   Purpose

   Template to generate via PCR a single gRNA for expression in S. cerevisiae

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti6/V5-p53_R273H

    Plasmid

    #22934

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R273H Mutant

    Available Since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB80-2xFKBP-L-mCherry-CAAX

    Plasmid

    #193713

    Purpose

    Rapalog-inducible coupling to plasma membrane (CAAX) via FKBP

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    2xFKBP-L-mCherry-CAAX
    

    Tags

    2xFKBP-mCherry

    Expression

    Mammalian

    Mutation

    mCherry: Met1 Deleted

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. EIF2AK1 gRNA (BRDN0001162524)

    Plasmid

    #77050

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EIF2AK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EIF2AK1 (EIF2AK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-EPHA3

    Plasmid

    #116733

    Purpose

    Lentiviral expression of EPHA3

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EPHA3 (EPHA3 Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET MBP mCherry LIC cloning vector (MBP-mCherry)

    Plasmid

    #29747

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

    Expression

    Bacterial

    Available Since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmr101A-cPr-mCherry (ErmR)

    Plasmid

    #177206

    Purpose

    Plasmid with p15A origin for constitutive mCherry expression in E. coli

    Depositor

    Alexey Merz

    Insert

    mCherry
    

    Expression

    Bacterial

    Promoter

    Synthetic constitutive promoter

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLG001 (empty vector)

    Plasmid

    #157879

    Purpose

    Empty vector backbone for defense systems

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    None
    

    Expression

    Bacterial

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. bZiPro

    Plasmid

    #86846

    Purpose

    express NS2B-NS3 protease in E.coli

    Depositor

    Dahai Luo

    Article

    Zhang et al Science. 2016 Dec 8. pii: aai9309.

    Inserts

    NS3 1-177
    NS2B45-96
    

    Tags

    a hexahistidine tag and a thrombin cleavage site

    Expression

    Bacterial

    Mutation

    NS2B cofactor region (residues 45–96) and NS3 pro…

    Promoter

    T7

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF-hSEMA4C-Fc

    Plasmid

    #74449

    Purpose

    Expresses human Semaphorin-4C ectodomain fused to human Fc in mammalian cells

    Depositor

    Roland Friedel

    Article

    Le et al Oncotarget. 2015 Mar 30;6(9):7293-304.

    Insert

    human Semaphorin-4C ectodomain with human Fc tag (SEMA4C Human)
    

    Tags

    human Fc tag

    Expression

    Mammalian

    Promoter

    EF1-alpha

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmPB3-VSV

    Plasmid

    #68039

    Purpose

    Expresses mouse Plexin-B3 with N-terminal VSV tag in mammalian cells

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Plexin-B3 (Plxnb3 Mouse)
    

    Tags

    VSV

    Expression

    Mammalian

    Available Since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEasyG3_nat

    Plasmid

    #184918

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge