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pcDNA3 barr1 HA
(Plasmid #14693)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14693 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    beta arrestin 1
  • Alt name
    arrestin 2
  • Alt name
    arrb1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1300
  • GenBank ID
    NM_012910
  • Entrez Gene
    Arrb1 (a.k.a. BARRES)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 barr1 HA was a gift from Robert Lefkowitz (Addgene plasmid # 14693 ; http://n2t.net/addgene:14693 ; RRID:Addgene_14693)
  • For your References section:

    Beta-arrestin-dependent formation of beta2 adrenergic receptor-Src protein kinase complexes. Luttrell LM, Ferguson SS, Daaka Y, Miller WE, Maudsley S, Della Rocca GJ, Lin F, Kawakatsu H, Owada K, Luttrell DK, Caron MG, Lefkowitz RJ. Science. 1999 Jan 29. 283(5402):655-61. 10.1126/science.283.5402.655 PubMed 9924018