|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12461||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneUBE2N (a.k.a. HEL-S-71, UBC13, UBCHBEN; UBC13, UbcH-ben, UbcH13)
/ Fusion Protein
- HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site EcoRV (destroyed during cloning)
- 5′ sequencing primer NA (Common Sequencing Primers)
EcoRV site was destroyed during cloning and replaced with a new NotI site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.0-HA-UbcH13 was a gift from Dong-Er Zhang (Addgene plasmid # 12461 ; http://n2t.net/addgene:12461 ; RRID:Addgene_12461)
For your References section:ISG15 modification of ubiquitin E2 Ubc13 disrupts its ability to form thioester bond with ubiquitin. Zou W, Papov V, Malakhova O, Kim KI, Dao C, Li J, Zhang DE. Biochem Biophys Res Commun. 2005 Oct 14. 336(1):61-8. 10.1016/j.bbrc.2005.08.038 PubMed 16122702