|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14808||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4244
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHes1 promoter
Insert Size (bp)1121
/ Fusion Protein
- GFPd2 (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
The Hes1 promoter is specific for progenitor cells in the developing retina. This promoter is also weakly active in Muller glia in the differentiated retina.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHes1-GFPd2 was a gift from Connie Cepko (Addgene plasmid # 14808 ; http://n2t.net/addgene:14808 ; RRID:Addgene_14808)
For your References section:Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T, Cepko CL. Proc Natl Acad Sci U S A. 2007 Jan 5. ():. 10.1073/pnas.0610155104 PubMed 17209010