|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15134||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerGary Nolan, Stanford
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Growth instructionsGrow in Stbl3 at 30C
Copy numberLow Copy
SpeciesR. norvegicus (rat)
Insert Size (bp)1100
MutationE43A, K44A, R47A
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Article Citing this Plasmid
Dominant negative Hes1.
Please note that the vector backbone pBABE-MN-IRES-E-GFP (also known as pBMN-I-GFP, Addgene plasmid# 1736) is extremely slow growing and may need 2 days to grow.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHes1-DN-GFP (CC#110) was a gift from Connie Cepko (Addgene plasmid # 15134 ; http://n2t.net/addgene:15134 ; RRID:Addgene_15134)
For your References section:rax, Hes1, and notch1 promote the formation of Muller glia by postnatal retinal progenitor cells. Furukawa T, Mukherjee S, Bao ZZ, Morrow EM, Cepko CL. Neuron. 2000 May . 26(2):383-94. 10.1016/S0896-6273(00)81171-X PubMed 10839357