pLIA mNIC myc (CC#341)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15131||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerCepko Lab
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameNotch1 (C-term)
Alt nameNotch 1
SpeciesM. musculus (mouse)
MutationCytoplasmic domain initiating at amino acid 1753. See author's map and article for more information.
Entrez GeneNotch1 (a.k.a. 9930111A19Rik, Mi, Mis6, N, N1, Ta, Tan1, lin, lin-12)
/ Fusion Protein
- myc (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
mNIC encodes the cytoplasmic domain of mouse Notch 1 with a myc tag at the N terminus.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLIA mNIC myc (CC#341) was a gift from Connie Cepko (Addgene plasmid # 15131 ; http://n2t.net/addgene:15131 ; RRID:Addgene_15131)
For your References section:The expression and function of Notch pathway genes in the developing rat eye. Bao ZZ, Cepko CL. J Neurosci. 1997 Feb 15. 17(4):1425-34. PubMed 9006984
Map uploaded by the depositor.