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pLIA (CC#1)
(Plasmid #15129)


Item Catalog # Description Quantity Price (USD)
Plasmid 15129 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Cepko Lab
  • Backbone size (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Tag / Fusion Protein
    • IRES-PLAP (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A replication-incompetent pLIA retroviral vector, based on the pBABE vector. In addition to the MMLV long terminal repeats, it also contains an internal ribosomal entry site (IRES) sequence derived from the encephalomyocarditis virus, in which the eleventh ATG of the IRES was fused in frame to the human placental alkaline phosphatase gene (PLAP).

pLIA has a cloning site for expressing exogenous genes under the transcriptional control of the LTR. The cloning site contains NotI, SfiI, SnaBI, and EcoRI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLIA (CC#1) was a gift from Connie Cepko (Addgene plasmid # 15129 ; ; RRID:Addgene_15129)
  • For your References section:

    The expression and function of Notch pathway genes in the developing rat eye. Bao ZZ, Cepko CL. J Neurosci. 1997 Feb 15. 17(4):1425-34. PubMed 9006984