pCLE
(Plasmid #17703)

• Purpose: (Empty Backbone)
• Depositing Lab: Nicholas Gaiano
• Publication: Gaiano et al Neuron. 2000 May . 26(2):395-404.

Backbone

• Vector backbone: pCLE
• Backbone size (bp): 7600
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: na

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pCLE includes a modified 5' LTR (CMV/MLV) to obtain higher titers, as well as the Xenopus EF1alpha enhancer/promoter to improve vector expression in the brain. In addition, the vector is dicistronic and utilizes an IRES to allow translation of the reporter gene, human placental alkaline phosphatase (PLAP).