|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17703||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 7600
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
pCLE includes a modified 5' LTR (CMV/MLV) to obtain higher titers, as well as the Xenopus EF1alpha enhancer/promoter to improve vector expression in the brain. In addition, the vector is dicistronic and utilizes an IRES to allow translation of the reporter gene, human placental alkaline phosphatase (PLAP).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCLE was a gift from Nicholas Gaiano (Addgene plasmid # 17703 ; http://n2t.net/addgene:17703 ; RRID:Addgene_17703)
For your References section:Radial glial identity is promoted by Notch1 signaling in the murine forebrain. Gaiano N, Nye JS, Fishell G. Neuron. 2000 May . 26(2):395-404. 10.1016/S0896-6273(00)81172-1 PubMed 10839358