pCLE NICD3
(Plasmid #26894)

• Depositing Lab: Nicholas Gaiano
• Publication: Dang et al Dev Neurosci. 2006;28(1-2):58-69.

Backbone

• Vector backbone: pCLE
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: May be slow-growing. Culturing for up to 48 hours under selection may be necessary to achieve robust growth
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NICD3
• Alt name: Notch3 intracellular domain
• Species: H. sapiens (human)
• Entrez Gene: NOTCH3 (a.k.a. CADASIL, CADASIL1, CASIL, IMF2, LMNS)
• Tags / Fusion Proteins:
  • IRES (C terminal on backbone)
  • Alkaline Phosphatase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: EF-1a-F

Resource Information

• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

EF1a xenopus enhancer-promoter-hNICD3-IRES-AlkPhos

A1757T mutation in Notch3 is not known to affect function.