|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26894||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Alt nameNotch3 intracellular domain
SpeciesH. sapiens (human)
Entrez GeneNOTCH3 (a.k.a. CADASIL, CADASIL1, CASIL, IMF2, LMNS)
/ Fusion Proteins
- IRES (C terminal on backbone)
- Alkaline Phosphatase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer EF-1a-F (Common Sequencing Primers)
EF1a xenopus enhancer-promoter-hNICD3-IRES-AlkPhos
A1757T mutation in Notch3 is not known to affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCLE NICD3 was a gift from Nicholas Gaiano (Addgene plasmid # 26894 ; http://n2t.net/addgene:26894 ; RRID:Addgene_26894)
For your References section:Notch3 signaling promotes radial glial/progenitor character in the mammalian telencephalon. Dang L, Yoon K, Wang M, Gaiano N. Dev Neurosci. 2006;28(1-2):58-69. 10.1159/000090753 PubMed 16508304