|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26891||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4800
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameNotch intracellular domain
SpeciesM. musculus (mouse)
Insert Size (bp)2500
Entrez GeneNotch1 (a.k.a. 9930111A19Rik, Mis6, N1, Tan1, lin-12)
/ Fusion Proteins
- FLAG (N terminal on insert)
- b-globin polyA (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer pCAG-Fwd (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAGGS-NICD was a gift from Nicholas Gaiano (Addgene plasmid # 26891 ; http://n2t.net/addgene:26891 ; RRID:Addgene_26891)
For your References section:Notch3 signaling promotes radial glial/progenitor character in the mammalian telencephalon. Dang L, Yoon K, Wang M, Gaiano N. Dev Neurosci. 2006;28(1-2):58-69. 10.1159/000090753 PubMed 16508304
Map uploaded by the depositor.