|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12205||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3500
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2000
Entrez GeneSTK3 (a.k.a. KRS1, MST2)
/ Fusion Protein
- Myc (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer SV40pro-F (Common Sequencing Primers)
Addgene's quality control sequence shows a mutation at amino acid E331D of STK3. This mutation is outside the kinase domain and the SARAH dimerization domain, and hence should not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJ3M-Mst2 was a gift from Jonathan Chernoff (Addgene plasmid # 12205 ; http://n2t.net/addgene:12205 ; RRID:Addgene_12205)
For your References section:The Ste20-like protein kinase, Mst1, dimerizes and contains an inhibitory domain. Creasy CL, Ambrose DM, Chernoff J. J Biol Chem. 1996 Aug 30. 271(35):21049-53. 10.1074/jbc.271.35.21049 PubMed 8702870