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(Plasmid #11344)


Item Catalog # Description Quantity Price (USD)
Plasmid 11344 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    L4440 is available at Addgene
  • Backbone size w/o insert (bp) 2790
  • Vector type
    Worm Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    This vector is propagated in E. coli strain DB3.1, due to the requirement to circumvent the lethality of the inherent ccdB gene until an insert is recombined into the Gateway cassette.
  • Copy number
    High Copy


  • Gene/Insert name
    gateway cassette

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site EcoRV (unknown if destroyed)
  • 3′ cloning site EcoRV (unknown if destroyed)
  • 5′ sequencing primer L4440 primer (AGCGAGTCAGTGAGCGAG)
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Andrew Fire created the vector backbone L4440
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gateway-modified RNAi feeding vector L4440. Use this vector for Gateway cloning of an RNAi target sequence. A Gateway recombinational cassette was placed into the EcoRV site of vector L4440 to generate this modified plasmid. To facilitate subcloning of a given DNA insert into this plasmid, Gateway recombinational attachment sites should be incorporated into primers used for amplificiation, as outlined in the Invitrogen Gateway manual. (Gateway is a registered trademark of Invitrogen Corporation.)

This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    L4440gtwy was a gift from Guy Caldwell (Addgene plasmid # 11344 ; ; RRID:Addgene_11344)