MMTV-SV40-Bssk
(Plasmid #1824)

• Purpose: (Empty Backbone)
• Depositing Lab: Philip Leder
• Publication: Ornitz et al Proc Natl Acad Sci U S A 1991 Feb 1;88(3):698-702.

Backbone

• Vector backbone: BlueScribe SK+
• Backbone size (bp): 7023
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: na

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest- BglII/ BglII+SalI
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

SalI to HindIII MMTV-LTR from PA9; SV40 PA/intron is from CDM8. Unique cloning sites for expression from the LTR are EcoRI and HindIII.
(Leder #C-197)