166,818 results

1. pJWV102-wtcas9sp

   Plasmid

   #99612

   Purpose

   Plasmid containing wild-type cas9sp

   Depositor

   Jan-Willem Veening, Morten Kjos

   Article

   van Raaphorst et al Proc Natl Acad Sci U S A. 2017 Jul 3. pi

   Insert

   cas9sp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PczcD

   Available Since

   Sept. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUC18-mini-Tn7T-Gm-lux

   Plasmid

   #64963

   Purpose

   mini-Tn7 luxCDABE transcriptional fusion vector

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Insert

   lux operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. peGFP C3-SPG20-PAAA

   Plasmid

   #218581

   Purpose

   Expresses GFP-tagged PPAY motif mutant (PAAA) of human SPG20 in mammalian cells; this mutant does not interact with ITCH

   Depositor

   Hemmo Meyer

   Article

   Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

   Insert

   SPG20 (SPART Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   changed Proline 172 to Alanine and Tyrosine 174 t…

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. peGFP C1-SPG20 SC domain

   Plasmid

   #218580

   Purpose

   Expresses GFP-tagged SC domain of human SPG20 in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

   Insert

   SPG20 (SPART Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-417

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHH0103_DYNLL1_Dynein-light

   Plasmid

   #110006

   Purpose

   Protein expression and purification of DYNLL1_Dynein-light

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   DYNLL1_Dynein-light (DYNLL1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEN_TT 3xFLAG-TWIST2

   Plasmid

   #197935

   Purpose

   Gateway entry vector for an inducible 3xFLAG-tagged TWIST2

   Depositor

   Kevin Janes

   Article

   Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

   Insert

   TWIST2 (TWIST2 Human)
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-ZIKVA-GFP-puro

   Plasmid

   #140090

   Purpose

   Fluorescence activatable reporter of ZIKV NS2B-NS3 protease activity

   Depositor

   Jorge Luis Arias-Arias

   Article

   Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

   Insert

   ZIKVA-GFP
   

   Use

   Lentiviral; Low expression

   Tags

   6XHis tag and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSPAsp-hp

   Plasmid

   #48122

   Purpose

   Shuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoter

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

   Insert

   predicted optimal signal peptide for Bacillus megaterium protein secretion
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-DENV2A-GFP-puro

   Plasmid

   #140089

   Purpose

   Fluorescence activatable reporter of DENV-2 NS2B-NS3 protease activity

   Depositor

   Jorge Luis Arias-Arias

   Article

   Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

   Insert

   DENV2A-GFP
   

   Use

   Lentiviral; Low expression

   Tags

   6XHis tag and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-FLEX-tdTomato

    Plasmid

    #28306

    Depositor

    Edward Boyden

    Has Service

    AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    tdTomato
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SSTR5-Tango

    Plasmid

    #66506

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    SSTR5 (SSTR5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

12. HDAC1 Flag

    Plasmid

    #13820

    Purpose

    Mammalian expression of human histone deacetylase 1 with flag tag

    Depositor

    Eric Verdin

    Article

    Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 9

    Insert

    HDAC1 (HDAC1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. C14orf169

    Plasmid

    #39114

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    C14orf169 (C14orf169 Human)
    

    Use

    Tags

    Tev His Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. mIFP-VASP-5

    Plasmid

    #56241

    Purpose

    Localization: Focal Adhesions, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    VASP (Vasp Mouse)
    

    Use

    Tags

    mIFP

    Expression

    Mammalian

    Mutation

    A209T in VASP

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pMRX-IP-EGFP-mouse Plaat3 (N157stop)

    Plasmid

    #168771

    Purpose

    Expresses EGFP-Plaat3 (C-terminal element is deleted) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

    Insert

    Hrasls3 (Plaat3 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMV-LEU2

    Plasmid

    #65334

    Purpose

    standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUs

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    LEU2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pU6-Sp-pegRNA-HEK3_CTT_ins

    Plasmid

    #132778

    Purpose

    S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    HEK3_CTT_ins pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT7-VP1SA11

    Plasmid

    #89162

    Purpose

    Express Rotavirus SA11 VP1 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FLAG-mBAF45c IRES GFP

    Plasmid

    #17882

    Depositor

    Jerry Crabtree

    Article

    Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

    Insert

    BAF45c (Dpf3 Mouse)
    

    Use

    Tags

    FLAG and IRES EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 13, 2008

    Availability

    Academic Institutions and Nonprofits only

20. FLAG-mBAF45b IRES GFP

    Plasmid

    #17881

    Depositor

    Jerry Crabtree

    Article

    Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

    Insert

    BAF45b (Dpf1 Mouse)
    

    Use

    Tags

    FLAG and IRES EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 13, 2008

    Availability

    Academic Institutions and Nonprofits only