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pAd PdxI-I-nGFP
(Plasmid #19411)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 19411 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAd CMV/V5 DEST
  • Backbone size w/o insert (bp) 34864
  • Vector type
    Adenoviral ; Entry Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Growth instructions
    Transform into TOP10 cells
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    pancreatic and duodenal homeobox 1
  • Alt name
    Pdx1, Ipf1; IDX-1; IPF-1; Mody4; STF-1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3200
  • Entrez Gene
    Pdx1 (a.k.a. IDX-1, IPF-1, Ipf1, Mody4, STF-1, pdx-1)
  • Tags / Fusion Proteins
    • IRES (C terminal on insert)
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site Gateway (unknown if destroyed)
  • 3′ cloning site Gateway (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAd PdxI-I-nGFP was a gift from Douglas Melton (Addgene plasmid # 19411)
  • For your References section:

    In vivo reprogramming of adult pancreatic exocrine cells to beta-cells. Zhou Q, Brown J, Kanarek A, Rajagopal J, Melton DA. Nature. 2008 Aug 27. ():. 10.1038/nature07314 PubMed 18754011