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pAd MafA-I-nGFP
(Plasmid #19412)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 19412 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAd CMV/V5 DEST
  • Backbone size w/o insert (bp) 34864
  • Vector type
    Adenoviral ; Entry Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Growth instructions
    Transform into TOP10 cells
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian)
  • Alt name
    Mafa
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2660
  • Entrez Gene
    Mafa (a.k.a. RIPE3b1)
  • Tags / Fusion Proteins
    • IRES (C terminal on insert)
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site Gateway (unknown if destroyed)
  • 3′ cloning site Gateway (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Qiuo Zhon
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene was unable to acquire sequence for this plasmid but is distributing the plasmid at the Melton Lab's request. Please address any problems with this plasmid to the Melton lab.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAd MafA-I-nGFP was a gift from Douglas Melton (Addgene plasmid # 19412 ; http://n2t.net/addgene:19412 ; RRID:Addgene_19412)
  • For your References section:

    In vivo reprogramming of adult pancreatic exocrine cells to beta-cells. Zhou Q, Brown J, Kanarek A, Rajagopal J, Melton DA. Nature. 2008 Aug 27. ():. 10.1038/nature07314 PubMed 18754011