pAd 2B Ngn3-I-nGFP
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19410||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepAd CMV/V5 DEST
- Backbone size w/o insert (bp) 34864
Vector typeAdenoviral ; Entry Vector
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsTransform into TOP10 cells
Copy numberLow Copy
Gene/Insert nameneurogenin 3
Alt nameNeurog3, Ngn3, Atoh5; Math4B
SpeciesM. musculus (mouse)
Insert Size (bp)2100
Entrez GeneNeurog3 (a.k.a. Atoh5, Math4B, bHLHa7, ngn3)
/ Fusion Proteins
- IRES (C terminal on insert)
- GFP (C terminal on insert)
- Cloning method Gateway Cloning
- 5′ cloning site Gateway (unknown if destroyed)
- 3′ cloning site Gateway (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byQiuo Zhon
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAd 2B Ngn3-I-nGFP was a gift from Douglas Melton (Addgene plasmid # 19410 ; http://n2t.net/addgene:19410 ; RRID:Addgene_19410)
For your References section:In vivo reprogramming of adult pancreatic exocrine cells to beta-cells. Zhou Q, Brown J, Kanarek A, Rajagopal J, Melton DA. Nature. 2008 Aug 27. ():. 10.1038/nature07314 PubMed 18754011
Map uploaded by the depositor.