pWPT-PDX1
(Plasmid #12256)

• Depositing Lab: Didier Trono
• Publication: Ritz-Laser et al Diabetologia. 2003 Jun . 46(6):810-21.

Backbone

• Vector backbone: pWPT
• Backbone size w/o insert (bp): 9560
• Vector type: Mammalian Expression, Lentiviral, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pdx1
• Species: H. sapiens (human)
• Entrez Gene: PDX1 (a.k.a. GSF, IDX-1, IPF1, IUF1, MODY4, PAGEN1, PDX-1, STF-1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: See map (not destroyed)
• 3′ cloning site: See map (not destroyed)
• 5′ sequencing primer: pBluescriptKS primer

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: Human PDX1 cDNA was provided by V. Schwitzgebel.
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.