|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12256||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9560
Vector typeMammalian Expression, Lentiviral, Cre/Lox
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsUse Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GenePDX1 (a.k.a. GSF, IDX-1, IPF1, IUF1, MODY4, PAGEN1, PDX-1, STF-1)
- Cloning method Restriction Enzyme
- 5′ cloning site See map (not destroyed)
- 3′ cloning site See map (not destroyed)
- 5′ sequencing primer pBluescriptKS primer (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pWPT-PDX1 was a gift from Didier Trono (Addgene plasmid # 12256 ; http://n2t.net/addgene:12256 ; RRID:Addgene_12256)
For your References section:Ectopic expression of the beta-cell specific transcription factor Pdx1 inhibits glucagon gene transcription. Ritz-Laser B, Gauthier BR, Estreicher A, Mamin A, Brun T, Ris F, Salmon P, Halban PA, Trono D, Philippe J. Diabetologia. 2003 Jun . 46(6):810-21. 10.1007/s00125-003-1115-7 PubMed 12783165