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pWPT-PDX1
(Plasmid #12256)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12256 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pWPT
  • Backbone size w/o insert (bp) 9560
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pdx1
  • Species
    H. sapiens (human)
  • Entrez Gene
    PDX1 (a.k.a. GSF, IDX-1, IPF1, IUF1, MODY4, PAGEN1, PDX-1, STF-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See map (not destroyed)
  • 3′ cloning site See map (not destroyed)
  • 5′ sequencing primer pBluescriptKS primer
  • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    Human PDX1 cDNA was provided by V. Schwitzgebel.
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWPT-PDX1 was a gift from Didier Trono (Addgene plasmid # 12256 ; http://n2t.net/addgene:12256 ; RRID:Addgene_12256)
  • For your References section:

    Ectopic expression of the beta-cell specific transcription factor Pdx1 inhibits glucagon gene transcription. Ritz-Laser B, Gauthier BR, Estreicher A, Mamin A, Brun T, Ris F, Salmon P, Halban PA, Trono D, Philippe J. Diabetologia. 2003 Jun . 46(6):810-21. 10.1007/s00125-003-1115-7 PubMed 12783165