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pEGFP-C1-hApg5
(Plasmid #22952)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22952 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Homo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae)
  • Alt name
    Atg5
  • Alt name
    Apg5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    830
  • Entrez Gene
    ATG5 (a.k.a. APG5, APG5-LIKE, APG5L, ASP, SCAR25, hAPG5)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-C1-hApg5 was a gift from Noboru Mizushima (Addgene plasmid # 22952 ; http://n2t.net/addgene:22952 ; RRID:Addgene_22952)
  • For your References section:

    A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy. Mizushima N, Sugita H, Yoshimori T, Ohsumi Y. J Biol Chem. 1998 Dec 18. 273(51):33889-92. 10.1074/jbc.273.51.33889 PubMed 9852036