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(Plasmid #39235)

Full plasmid sequence is not available for this item.



This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39235 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 8100
  • Modifications to backbone
    pChREBP was generated by RT-PCR from MIN6 cell total RNA using the following primers: 5′-ATG GAA CAG AAG CTT ATT TCT GAA GAA GAT CTT GCG CGC GCG CTG GCG GAT CTA TCC GTG AAC-3′ (c-myc sequence underlined) and 5′-CGC GGA GCT CTT ATA ATG GTC TCC CCA GGG TGC CCT CTG TGA CTG C-3′. The resulting cDNA was phosphorylated by T4 polynucleotide kinase (Roche) and subcloned into pcDNA3 (Invitrogen) vector linearized with EcoRV.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Mlxipl (a.k.a. ChREBP, Mlx, WS-bHLH, Wbscr14, bHLHd14)
  • Promoter CMV
  • Tag / Fusion Protein
    • cMyc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pChREBP was a gift from Isabelle Leclerc (Addgene plasmid # 39235 ; ; RRID:Addgene_39235)
  • For your References section:

    ChREBP binding to fatty acid synthase and L-type pyruvate kinase genes is stimulated by glucose in pancreatic beta-cells. da Silva Xavier G, Rutter GA, Diraison F, Andreolas C, Leclerc I. J Lipid Res. 2006 Nov;47(11):2482-91. Epub 2006 Aug 4. 10.1194/jlr.M600289-JLR200 PubMed 16891625