pChREBP
(Plasmid #39235)

• Depositing Lab: Isabelle Leclerc
• Publication: da Silva Xavier et al J Lipid Res. 2006 Nov;47(11):2482-91. Epub 2006 Aug 4.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 8100
• Modifications to backbone: pChREBP was generated by RT-PCR from MIN6 cell total RNA using the following primers: 5′-ATG GAA CAG AAG CTT ATT TCT GAA GAA GAT CTT GCG CGC GCG CTG GCG GAT CTA TCC GTG AAC-3′ (c-myc sequence underlined) and 5′-CGC GGA GCT CTT ATA ATG GTC TCC CCA GGG TGC CCT CTG TGA CTG C-3′. The resulting cDNA was phosphorylated by T4 polynucleotide kinase (Roche) and subcloned into pcDNA3 (Invitrogen) vector linearized with EcoRV.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ChREBP
• Alt name: Mlxipl
• Species: M. musculus (mouse)
• Entrez Gene: Mlxipl (a.k.a. ChREBP, Mlx, WS-bHLH, Wbscr14, bHLHd14)
• Promoter: CMV
• Tag / Fusion Protein:
  • cMyc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: EcoRV (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry