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pcDNA3.1-2xFLAG-SREBP-1c
(Plasmid #26802)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26802 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1+
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SREBP-1c
  • Alt name
    SREBP1
  • Alt name
    SREBP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1398
  • Entrez Gene
    SREBF1 (a.k.a. SREBP1, bHLHd1)
  • Tag / Fusion Protein
    • 2XFLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

See associated publication for details of SREBP-1c isoform

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-2xFLAG-SREBP-1c was a gift from Timothy Osborne (Addgene plasmid # 26802 ; http://n2t.net/addgene:26802 ; RRID:Addgene_26802)
  • For your References section:

    Selective coactivator interactions in gene activation by SREBP-1a and -1c. Toth JI, Datta S, Athanikar JN, Freedman LP, Osborne TF. Mol Cell Biol. 2004 Sep . 24(18):8288-300. 10.1128/MCB.24.18.8288-8300.2004 PubMed 15340088