Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #26807)


Item Catalog # Description Quantity Price (USD)
Plasmid 26807 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Sterol Regulatory Element Binding Protein 2
  • Alt name
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    L7V, G76S, K395R and I482L
  • Entrez Gene
    SREBF2 (a.k.a. SREBP-2, SREBP2, bHLHd2)
  • Promoter CMV
  • Tag / Fusion Protein
    • 2x FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid expresses the truncated mature SREBP-2 protein (aa 1-482) which is targeted to the nucleus where it activates gene expression and not the full length SREBP-2 inactive precursor protein that is tethered to the endoplasmic reticulum. The plasmid was cloned similarly to the SREBP-1a and 1c constructs that are described in the publication listed below.

Please note that the SREBP-2 sequence contains L7V, G76S, K395R and I482L when compared to GenBank entry NP_004590.2. These changes may represent natural variants and have no known functional consequences. This is the exact plasmid used in the associated article listed below.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-2xFLAG-SREBP-2 was a gift from Timothy Osborne (Addgene plasmid # 26807 ; ; RRID:Addgene_26807)
  • For your References section:

    Selective coactivator interactions in gene activation by SREBP-1a and -1c. Toth JI, Datta S, Athanikar JN, Freedman LP, Osborne TF. Mol Cell Biol. 2004 Sep . 24(18):8288-300. 10.1128/MCB.24.18.8288-8300.2004 PubMed 15340088