pcDNA3.1-2xFLAG-SREBP-2
(Plasmid #26807)

• Depositing Lab: Timothy Osborne
• Depositing Organization: Sanford Burnham Prebys Medical Discovery Institute
  Located in United States of America
• Publication: Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-300.
• Citations: 20 Articles

Backbone

• Vector backbone: pcDNA3.1+
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sterol Regulatory Element Binding Protein 2
• Alt name: SREBP-2
• Alt name: SREBF2
• Alt name: SREBP2
• Species: H. sapiens (human)
• Insert Size (bp): 1440
• Mutation: L7V, G76S, K395R and I482L
• Entrez Gene: SREBF2 (a.k.a. SREBP-2, SREBP2, bHLHd2)
• Promoter: CMV
• Tag / Fusion Protein:
  • 2x FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Sanford-Burnham Medical Research Institute Ancillary Agreement
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid expresses the truncated mature SREBP-2 protein (aa 1-482) which is targeted to the nucleus where it activates gene expression and not the full length SREBP-2 inactive precursor protein that is tethered to the endoplasmic reticulum. The plasmid was cloned similarly to the SREBP-1a and 1c constructs that are described in the publication listed below.

Please note that the SREBP-2 sequence contains L7V, G76S, K395R and I482L when compared to GenBank entry NP_004590.2. These changes may represent natural variants and have no known functional consequences. This is the exact plasmid used in the associated article listed below.

DNA (Catalog # 26807-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Sanford-Burnham Medical Research Institute Ancillary Agreement
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 26807-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Sanford-Burnham Medical Research Institute Ancillary Agreement
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.