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pcDNA3.1-2xFLAG-SREBP-2
(Plasmid #26807)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26807 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1+
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sterol Regulatory Element Binding Protein 2
  • Alt name
    SREBP-2
  • Alt name
    SREBF2
  • Alt name
    SREBP2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1440
  • Mutation
    L7V, G76S, K395R and I482L
  • Entrez Gene
    SREBF2 (a.k.a. SREBP-2, SREBP2, bHLHd2)
  • Promoter CMV
  • Tag / Fusion Protein
    • 2x FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid expresses the truncated mature SREBP-2 protein (aa 1-482) which is targeted to the nucleus where it activates gene expression and not the full length SREBP-2 inactive precursor protein that is tethered to the endoplasmic reticulum. The plasmid was cloned similarly to the SREBP-1a and 1c constructs that are described in the publication listed below.

Please note that the SREBP-2 sequence contains L7V, G76S, K395R and I482L when compared to GenBank entry NP_004590.2. These changes may represent natural variants and have no known functional consequences. This is the exact plasmid used in the associated article listed below.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-2xFLAG-SREBP-2 was a gift from Timothy Osborne (Addgene plasmid # 26807 ; http://n2t.net/addgene:26807 ; RRID:Addgene_26807)
  • For your References section:

    Selective coactivator interactions in gene activation by SREBP-1a and -1c. Toth JI, Datta S, Athanikar JN, Freedman LP, Osborne TF. Mol Cell Biol. 2004 Sep . 24(18):8288-300. 10.1128/MCB.24.18.8288-8300.2004 PubMed 15340088