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(Plasmid #60444)


Item Catalog # Description Quantity Price (USD)
Plasmid 60444 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pGL2 basic
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5357
  • Total vector size (bp) 5504
  • Modifications to backbone
    Contains the hamster HMG-CoA synthase promoter sequence harboring SREBP responsive region (-324/-225) fused directly to minimal HMG-CoA sythase TATA box (-28/+39) that is linked to luciferase in polylinker of pGL2 basic
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    HMG-CoA synthase promoter (-324/-225)
  • Alt name
    3-Hydroxy-3-methylglutaryl-coenzyme A synthase promoter
  • Species
  • Insert Size (bp)
  • Promoter HMG-CoA synthase (-324/-225) & TATAA (-28/+39)
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SstI or SacI (not destroyed)
  • 3′ cloning site HindIII for promoter and TATAA; NheI for promoter region only (not destroyed)
  • 5′ sequencing primer GLprimer1
  • 3′ sequencing primer GLprimer2
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSynSRE-T-Luc was a gift from Timothy Osborne (Addgene plasmid # 60444 ; ; RRID:Addgene_60444)
  • For your References section:

    Sterol regulation of 3-hydroxy-3-methylglutaryl-coenzyme A synthase gene through a direct interaction between sterol regulatory element binding protein and the trimeric CCAAT-binding factor/nuclear factor Y. Dooley KA, Millinder S, Osborne TF. J Biol Chem. 1998 Jan 16;273(3):1349-56. PubMed 9430668